Electrospray mass spectrometry on a sector instrument for the analysis of small oligoribonucleotides
For the antisense and ribozyme approach the sensitive analysis of small modified oligonucleotides is needed. While the molecular weight may confirm the presence of a modification, reproducible fragmentation schemes are needed to identify the modification side. ESI nozzle-skimmer fragmentation is studied on a EBqQ instrument for the fragmentation scheme of small oligoribonucleotides. Since electrospray is a concentration sensitive ionisation method, the flow rate was decreased to 1 micro l/min without losing sensitivity using a triple layer design. SF(6) was used as sheath gas to prevent discharging. Thus, analysis could be performed in the low picomol range. With nozzle-skimmer fragmentation (1,2) sequence specific fragments are generated. This method may assist in the synthesis of modified oligonucleotides.
Bibliographic Reference: Paper presented: 43rd ASMS Conference on Mass Spectrometry and Allied Topics, Atlanta, Georgia (US), May 21-26, 1995
Availability: Available from (1) as Paper EN 38976 ORA
Record Number: 199510709 / Last updated on: 1995-07-07
Original language: en
Available languages: en