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For the antisense and ribozyme approach the sensitive analysis of small modified oligonucleotides is needed. While the molecular weight may confirm the presence of a modification, reproducible fragmentation schemes are needed to identify the modification side. ESI nozzle-skimmer fragmentation is studied on a EBqQ instrument for the fragmentation scheme of small oligoribonucleotides. Since electrospray is a concentration sensitive ionisation method, the flow rate was decreased to 1 micro l/min without losing sensitivity using a triple layer design. SF(6) was used as sheath gas to prevent discharging. Thus, analysis could be performed in the low picomol range. With nozzle-skimmer fragmentation (1,2) sequence specific fragments are generated. This method may assist in the synthesis of modified oligonucleotides.

Additional information

Authors: WOLTER M A, JRC Ispra (IT);MONTANARELLA L, JRC Ispra (IT);FACCHETTI S, JRC Ispra (IT);ENGELS J W, Johann Wolfgang Goethe Universität, Institute for Organic Chemistry, Frankfurt (DE)
Bibliographic Reference: Paper presented: 43rd ASMS Conference on Mass Spectrometry and Allied Topics, Atlanta, Georgia (US), May 21-26, 1995
Availability: Available from (1) as Paper EN 38976 ORA
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