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A large number of studies have been carried out in recent years on the intercellular communication mediated by gap junctions. This cellular function seems to be essential in tissue homeostasis for effective cellular control. This study reports on the cytootoxicity of thirteen tumour promoters belonging to different structural classes (phorbol esthers, fatty acids, polychlorinated biphenyls, peroxides and antioxidants) using short- and long-term tests on two murine cell lines (3T3-L1 and A31-1-1). This study demonstrated that the three colorimetric tests show good correlation, that the two murine cell lines exhibit different sensibilities at 24 h, 72 h and 7 days' exposure, and that the mean IC50 values sampled using the three colorimetric tests at 72 h range from 0.010 mM to 4.63 mM for the A31-1-1 murine cell line and from 0.022 mM to 4.4 mM for the 3T3-L1 murine cell line.

Additional information

Authors: GRIBALDO L, JRC Ispra (IT);MILANI R, JRC Ispra (IT);BALLS M, JRC Ispra (IT)
Bibliographic Reference: Paper presented: The 2nd Italian Symposium on Molecular and Cellular Toxicology, Genova (IT), April 14-16, 1994
Availability: Available from (1) as Paper IT 38206 ORA
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