Purine enzyme profile in human colon carcinoma cell lines and differential sensitivity to deoxycoformycin and 2'-deoxyadenosine in combination
Different cell lines, two from human colon carcinoma (LoVo and HT29) and one from Chinese hamster ovary (CHO K-1), were examined to assess the effect of deoxycoformycin (dCF), an inhibitor of adenosine deaminase (ADA), and 2'-deoxyadenosine (dAdo) on their growth. When used alone, neither dCF nor dAdo were cytotoxic for the three cell lines, while their combination caused inhibition of cell growth, with the following sensitivity: CHO K-1 > LoVo > HT29. The pattern of enzymatic activities involved in the metabolism of dAdo in the three cell lines was also studied. The phosphorylation of dAdo by adenosine kinase appears to play a central role in the toxicity of the deoxynucleoside in combination with dCF.
Bibliographic Reference: Article: International Journal of Cancer (1995)
Record Number: 199511173 / Last updated on: 1995-08-23
Original language: en
Available languages: en