Early toxic effects detected by apoptosis on murine bone marrow cells and established cell lines
In the frame of studies on in vitro models for testing cytotoxic effects of chemicals and drugs on hematopoietic cell populations, whole murine bone marrow cells (BMC) and two established murine cell lines, SR-4987 (a stromal cell line) and WEHI-3B (a murine myelomonocytic leukemia), were exposed to etopoxide (EPT) and doxorubicin (DXR). The toxic effects were measured after 1, 5 and 24 hours of exposure, by two different endpoints: viability by MTT reduction and apoptosis by Elisa DNA fragmentation test. Apoptosis detection by the Elisa test on SR-4987 was lower than apoptosis in BMC but is was already detectable in cell lysates at 5 hours with non-cytotoxic doses of ETP and DXR. In BMC, following 5 hours of exposure, ETP caused little increase in apoptosis; this increased four-fold by 24 hours. With DXR, apoptosis was also readily detectable at 5 hours. WEHI-3B exhibited a detectable increase of apoptosis in the supernatant at 24 hours of exposure to DXR and at 6 hours of treatment with ETP in the lysate. Results suggest that the evaluation of apoptosis at 5 hours after exposure, rather than determining cytotoxicity using the MTT method, is the best way to identify early toxic effects on both the bone marrow cells and on the two established cell lines.
Bibliographic Reference: Paper presented: June, Lecce (IT)
Availability: Available from (1) as Paper EN 39106 ORA
Record Number: 199511259 / Last updated on: 1995-10-13
Original language: en
Available languages: en