Electrochemical study of the binding properties of a metallothionein I related peptide with cadmium and/or zinc
An electrochemical study was performed, using differential pulse polarography, of the binding properties of the peptide Lys-Cys-Thr-Cys-Ala Thionein Fragment [56-61] MT I(FT) with cadmium and zinc. This molecule, intrinsic to the metallothionein structure, was chosen as a model of the Cd, Zn metallothioneins' ion exchange and binding properties. The influence of the addition of metal ions, cadmium and/or zinc, was studied with different ratios of complexing ligand/metallic cations, FT/M (II). The equilibrium concerning the complexation equilibrium of formation and dissociation was investigated as a function of pH, going from acid to basic solution and vice versa in different mixtures: Cd:FT, Zn:FT and Cd:Zn:FT. The apparent stability constants of the complexes Cd-FT, Zn-FT were estimated at different pH values.
Bibliographic Reference: Article: Electroanalysis (1995)
Record Number: 199511503 / Last updated on: 1995-11-03
Original language: en
Available languages: en