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Abstract

Reversed-phase high performance liquid chromatography (HPLC) with ultraviolet (UV) detection makes the separation of metallothionein (MT) isoforms possible. Adding electrochemical detection to UV detection in series, is an efficient tool for characterization of the different peaks, allowing the distinction of sulfhydryl (SH) containing compounds (isoMT) from other compounds such as those having disulphide bonds in their structure.

Each MT exhibits a different polymorphism, the number of separated species ranging from 1 to about 6. The detection of some peaks depends on the experimental conditions, mainly the sample preparation pH and the storage time. On the whole, this study demonstrates the difference in the degree of reactivity of the various MT's. The analytical characteristics of the electrochemical detection, detection limits and range of linearity of the response, have been determined. .

Additional information

Authors: CORDEIRO RAPOSO F, JRC Geel (BE);BORDIN G, JRC Geel (BE);RODRIGUEZ A, JRC Geel (BE)
Bibliographic Reference: Paper presented: Vth Comtox Symposium on Toxicology and Clinical Chemistry of Metals, Vancouver (CA), July 10-13, 1995
Availability: Available from (1) as Paper EN 39130 ORA
Record Number: 199512035 / Last updated on: 1996-01-15
Category: PUBLICATION
Original language: en
Available languages: en