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We have investigated the induction of gene mutations by pyrene-1,6-quinone in V79 cells which lack the capacity to oxidatively metabolize many foreign chemicals to mutagenic products, and in nearly-diploid Chinese hamster epithelial liver (CHEL) cells which retain xenobiotic-metabolizing enzyme activities. The experiments were carried out in the presence and in the absence of dicoumarol, a potent inhibitor of the flavoprotein DT-diaphorase.

In V79 cells the quinone was cytotoxic and mutagenic over a narrow range of concentrations. In CHEL cells P-1.6-quinone was equally cytotoxic but not mutagenic. When dicoumarol was added to the CHEL cell cultures, the cytotoxicity was increased and the mutation frequency increased. In CHEL cells, P-1,6-quinone may be reduced by 2-electron reduction (via DT-diaphorase) to its corresponding hydroquinone and then conjugated with glucuronic acid or sulfate. .

Additional information

Authors: TURCHI G, JRC Ispra (IT);ROSSINI P, JRC Ispra (IT);GLATT H R, German Institute for Human Nutrition (DE)
Bibliographic Reference: Paper presented: 15th International Symposium on Polycyclic Aromatic Compounds, Belgirate (IT), September 19-22, 1995
Availability: Available from (1) as Paper EN 39385 ORA
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