Sensitivity of C3H1OT1/2 cells to radiation-induced killing and neoplastic transformation as a function of cell cycle
Cell age sensitivity to both cell killing and neoplastic transformation induced by radiation was investigated using synchronised populations of C3H10T1/2 cells. Mitotic suspensions, collected using a mitotic shake-off procedure, were irradiated with 4 Gy of 250 kVp X-rays or 0.5 Gy of fission neutrons from the RSV-TAPIRO reactor at CR-Casaccia. For study of cell killing the mitotic cell suspensions were either irradiated immediately after collection, or plated for subsequent irradiation. The response pattern observed is similar after X-rays and neutron irradiation, but the magnitude of the variation through the cell cycle is smaller in the case of neutrons. For study of neoplastic transformation induction the irradiation was performed immediately after collection (ie in M phase, or at mid G1, G1/S and G2 phases). The sensitivity of the G2/M phase was examined by irradiating the cells with 4 Gy of X-rays. The transformation frequency shows a small variation after neutron irradiation.
Bibliographic Reference: Article : International Journal of Radiation Biology (1995)
Record Number: 199512144 / Last updated on: 1996-01-15
Original language: en
Available languages: en