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Oxidative hair dyeing plays an important role with respect to changing the colour of human hair. Most of the dye forming substances, the dye intermediates, are of aromatic nature. With the aim of establishing an analytical method for the separation and quantification of the dye intermediates, the chromatographic behaviour of a selection of these products was investigates. The separation was done by high performance liquid chromatography (HPLC), using reversed-phase chromatography. Ultraviolet (UV) and diode array detection were employed. A quantitative separation procedure was established with which it is possible to separate and identify intermediates according to their retention times and their UV spectra.

The influence of light, temperature and antioxidants on the chromatographic behaviour of the substances was recorded. It was shown that their influence on the stability of the different substances varied. Conditions for the analytical separation of hair dye intermediates must be very narrowly defined in order to obtain reproducible results.

Additional information

Authors: PEL E, JRC Geel (BE);BORDIN G, JRC Geel (BE);RODRIGUEZ A, JRC Geel (BE)
Bibliographic Reference: Article: Journal of Liquid Chromatography
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