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Abstract

The work described in this report is part of a project to improve methodology and to prepare certified reference materials for the determination of ochratoxin A. This report describes the results of the fourth phase of this project, the first intercomparison of methods for the determination of ochratoxin A in pig kidney material. Twenty laboratories from eight European Union member states took part in the intercomparison study to determine the ochratoxin A content of a blank pig kidney material and a material naturally contaminated at a level of between 5 mg kg{-1} and 10mg kg {-1} ochratoxin A. The materials were freeze dried and were reconstituted prior to analysis. The participants used a variety of methods including several different extraction solvents and clean up procedures. All except two laboratories detected ochratoxin A in the blank material, with the mean of the mean results being 0.52 mg kg{-1} for the reconstituted material. Almost all laboratories reported lower than usual recoveries for the freeze dried material. Recoveries ranged from 43% to 128%. The mean +/- standard deviation of the mean results for the contaminated material following recovery correction was 9.7 mg kg{-1} +/- 3.41 mg kg{-1} for the reconstituted material with a range of 5.7 mg kg{-1} to 22.8 mg kg{-1}.

Additional information

Authors: ENTWISLE A C, Leatherhead Food Research Association (GB);WILLIAM A C, Leatherhead Food Research Association (GB);FARNELL P J, Leatherhead Food Research Association (GB);JØRGENSEN K, National Food Agency of Denmark, Søborg (DK);BOENKE A, CEC Bruxelles (BE);J?RGENSEN K, National Food Agency of Denmark, S?borg (DK)
Bibliographic Reference: EUR 16879 EN (1996) 51pp., FS, free of charge
Availability: Available from the European Commission, DG XII-C-5, 200 rue de la Loi, B-1049 Bruxelles (BE)
ISBN: ISBN 92-827-6640-3
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