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The thylakoid bioassay has been developed to determine the effect of photosystem II specific herbicides in water on the basis of light-dependant oxygen evolution and inhibition of fluorescence yield of thylakoids which are used as a biological unit. In this paper we present the first results of the lyophilization of thylakoids. The application of freeze-dried thylakoids makes the bioassay faster and easy to handle. The main requirements are long-term stability of the biological unit and preservation of its photosynthetic activity without reducing the sensitivity of the bioassay. The technique maintains the photosynthetic activity after lyophilization at 82 +/- 9% of its original level.

Additional information

Authors: ZIMMERMANN G M, JRC Geel (BE);KRAMER G N, JRC Geel (BE);SCHNABL H, Institut für Landwirtschaftliche Botanik de Rheinischen Friedrich-Wilhelms-Universität Bonn (DE)
Bibliographic Reference: Article: Environmental Toxicology and Chemistry (1996)
Record Number: 199610472 / Last updated on: 1996-05-20
Original language: en
Available languages: en