DT-Diaphorase affects the mutagenic activity of pyrene 1,6-quinone in Chinese hamster epithelial liver cells
The mutagenic potential of pyrene 1,6-quinone has been studied in a wide range of in vitro genetic assays using mammalian cell lines. Pyrene 1,6-quinone induced gene mutations and micronuclei in V79 cells whereas in CHEL cells, an epithelial liver cell line which retained activities of various xenobiotic-metabolizing enzymes, an non-specific pattern of structural and numerical chromosomal aberrations were observed. In this study we have evaluated the mutagenic activity of pyrene 1,6-quinone on V79 and CHEL cells in the presence and absence of the dicoumarol, a potent inhibitor of DT-diaphorase. In V79 cells dicoumarol (100 mM) did not affect the mutagenic response, whereas in CHEL cells the mutation frequency significantly increased to suggest the possible role of DT-diaphorase, expressed in the liver cells at high levels, in the detoxification of pyrene, 1,6-quinone to redox-stable hydroquinone.
Bibliographic Reference: Article: ATLA, UK (1996)
Record Number: 199610474 / Last updated on: 1996-05-20
Original language: en
Available languages: en