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  • Characterisation of Metallothionein Isoforms by Reversed-phase High Performance Liquid Chromatography with On-line UV and Electrochemical Detection

Abstract

Four metallothioneins (MT), rabbit liver MT (RL), MT-1 (RL-1) and MT-2 (RL 2) and horse kidney MT (HK), were subjected to reversed phase high performance liquid chromatography (HPLC) with online ultraviolet (UV) and electrochemical (EC) detection, the latter comprising of a graphite electrode. The MTs are found to exhibit a different polymorphism and the various peaks differ in their detectability depending on the detection mode. We can assume that the peaks detected by both EC and UV modes correspond to the original thiol containing isoforms, while those less hydrophobic of RL 1 and RL correspond to modified species, probably containing disulphide bonds which explains the lack of EC response at the graphite electrode. Since many MTs are highly reactive, this UV/EC combination appears to be a very good tool for a quick identification of reduced (thiolic) isoforms from oxidised (with disulphides) species.

Additional information

Authors: BORDIN G, JRC Geel (BE);CORDEIRO RAPOSO F, JRC Geel (BE);RODRIGUEZ A, JRC Geel (BE)
Bibliographic Reference: Article: Journal of Liquid Chromatography, (1996)
Record Number: 199610541 / Last updated on: 1996-05-27
Category: PUBLICATION
Original language: en
Available languages: en