Factors influencing the Separation of Metallothionein Isoforms by CZE using an Uncoated Capillary Column
Metallothionein (MT) isoforms are readily separated by capillary zone electrophoresis (CZE) with tris(hydroxymethyl) aminomethane (TIRS) borate buffer using an uncoated polyimide cladded fused silica capillary column. The effects of buffer concentration and composition, pH, running voltage and temperature on the electroosmotic mobility and electrophoretic mobilities of the model samples (rabbit liver MT and horse kidney MT) were investigated. Also the effect of organic modifiers on the separation of metallothionein isoforms is described. With organic modifiers it is possible to obtain a good separation for the entire electropherogram of rabbit liver MT in one run rather than 2 separate runs. The effects of the organic modifers were found to be modifer specific although the trends were in the same direction (ie decreasing electroosmotic mobility with increasing percentage of organic modifier).
Bibliographic Reference: Paper presented: 18th International Symposium on Capillary Chromatogrpahy, Riva del Garda (IT), May 20-24, 1996
Availability: Available from (1) as Paper EN 39603 ORA
Record Number: 199610599 / Last updated on: 1996-05-27
Original language: en
Available languages: en