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We present here the results obtained within the framework of an EU funded project aimed to develop and validate alternative metabolic activating systems to be used in short-term mutagenicity assays, in order to reduce the use of laboratory animals for toxicology testing. The activating systems studied were established cell lines (Hep G2, CHEL), genetically engineered V79 cell lines expressing specific rat cytochromes P450, crythrocyte-derived systems, CYP-mimetic chemical systems and plant homogenates. The metabolically competent cell lines were used as indicator cells for genotoxic effects as well as for the preparation of external activating systems using other indicator cells.

Additional information

Authors: RUEFF J ET AL, UNL, Department of Genetics, Lisbon (PT);CHIAPELLA ET AL, Autonomous University of Barcelona, Department of Genetics and Microbiology (ES);CHIPMAN J K, University of Birmingham, School of Biochemistry (GB);DARROUDI F ET AL, State University of Leiden-Wassenaarseweg, Department of Radiation Genetics and Chemical Mutagenesis (NL);DUVERGER-VAN BOGAERT M ET AL, Catholic University of Louvain, Unit of Turatogenesis and Mutagenesis, Brussels (BE);FONTI ET AL, University of Tuschia, Department of Agrobiology and Agrochemistry, Viterbo (IT);WERLE-SCHNEIDER G ET AL, University of Mainz, Department of Toxicology (DE);TURCHI G, JRC Ispra (IT)
Bibliographic Reference: Article: Mutation Research, Vol. 353 (1996) pp. 151-176
Record Number: 199611400 / Last updated on: 1996-12-18
Original language: en
Available languages: en