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An analytical method based on gas-chromatography-mass-spectrometry (GC-MS) analysis was developed as a fast screening tool to verify the authenticity of extracts from different Echinacea roots (Echinacea angustifolia, Echinacea pallida, Echinacea purpurea). Various extraction methods (ie soxhlet extraction, supercritical fluid extraction (SFE)), and maceration with three different solvents were applied and the extracts were analysed by GC-MS. The registered chromatograms were evaluated by multivariate data analysis (ie cluster analysis, principal component analysis and discriminant analysis) to reveal whether a classification into the three main Echinacea species is possible with obtained information. GC-MS analysis of Echinacea extracts together with multivariate data analysis displayed substantial classification power, as a good separation of the three different species was achieved. This analytical approach was not only suitable for classification, but was also robust enough that no distortion by aging of root samples occurred.

Additional information

Authors: LIENERT D, JRC Ispra (IT);ANKLAM E, JRC Ispra (IT);PANNE U, Institute of Hydrochemistry, Munich (DE)
Bibliographic Reference: Article: Photochemical Analysis
Record Number: 199710771 / Last updated on: 1997-07-02
Original language: en
Available languages: en