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  • Speciation of organic and inorganic selenium in a biological CRM based on microbore ion exchange chromatography coupled to ICP-AES via a direct injection nebulizer or coupled to ETAAS

Abstract

A speciation method for selenomethionine, selenite, selenate, selenocystine,and trimethylselenonium is described. It is based on anion exchange chromatographic separation using two different metal-free microbore anion exchange columns followed by inductively coupled plasma-atomic emission spectroscopy (ICP-AES) for detection of selenium. The chromatographic eluent is nebulized into the plasma at a flow rate of 80 ml min{-1} using a direct injection nebulizer (DIN). It is possible to separate selenomethionine, selenite, selenate and selenocystine within five minutes. The method was then applied to sample extracts from CRM 402, a certified reference material of biological origin certified for a high total concentration of selenium. This material is a lyophilized powder of white clover grown on selenium enriched soil.

Additional information

Authors: EMTEBORG H, JRC Geel (BE);BORDIN G, JRC Geel (BE);RODRIGUEZ A R, JRC Geel (BE)
Bibliographic Reference: Article: The Analyst, Royal Society of Chemistry (1997)
Record Number: 199810127 / Last updated on: 1998-02-12
Category: PUBLICATION
Original language: en
Available languages: en