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In this study the electrochemical characteristics of metallothioneins have been widely investigated using cyclic voltammetry with a hanging mercury drop electrode in order to elucidate the diffusion-adsorption processes under different experimental conditions. Two commercially available metallothioneins of animal origin have been used. The assignment of electrochemical peaks has been made according to results obtained using different polarographic and voltammetric techniques. Two different systems have been distinguished: the oxidation of the mercury electrode in the presence of thiol groups of metallothionein and the reduction of metal ions complexed by the molecule. The influence of the buffer concentration, scan range, number of cycles, scan rate and concentration was investigated.

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Authors: HARLYK C, JRC Geel (BE);BORDIN G, JRC Geel (BE);RODRIGUEZ A R, JRC Geel (BE);NIETO O, University of Vigo (ES)
Bibliographic Reference: Paper presented: ESEAC 98, Coimbra (PT), May 24-28, 1998
Availability: Available from (1) as Paper EN 41490 ORA
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