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Abstract

This standard describes a method for the detection of a bovine specific mitochondrial deoxyribonucleic acid (mtDNA) sequence in ruminant feeds. The whole procedure comprises the following steps:

- Deoxyribonucleic acid (DNA) extraction;
- Amplification of a bovine mtDNA by polymerase chain reaction (PCR) technique;
- Confirmation of the bovine-specific amplification by means of gel electrophoresis.
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Additional information

Authors: VON HOLST C, JRC Ispra (IT);LIPP M, JRC Ispra (IT);ANKLAM E, JRC Ispra (IT);TARTAGLIA M, Istituto Superiore di Sanità, Laboratorio di Medicina Veterinaria, Roma (IT);MACRI A, Istituto Superiore di Sanità, Laboratorio di Medicina Veterinaria, Roma (IT)
Bibliographic Reference: EUR 18096 EN (1998) 12pp., free of charge
Availability: Available from the Public Relations and Publications Unit, JRC Ispra, I-21020 Ispra (IT), Fax: +39-0332-785818
Record Number: 199811077 / Last updated on: 1998-09-28
Category: PUBLICATION
Original language: en
Available languages: en
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