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  • The influence of experimental conditions and of organic solvents as modifiers on the separation of metallothionein isoforms by capillary zone electrophoresis in an uncoated capillary column.

Abstract

Putative metallothionein (MT) isoforms are readily separated by capillary zone electrophoresis (CZE) with tris(hydroxymethyl)aminomethane (Tris) - borate buffer using an uncoated polyimide cladded fused-silica capillary column. The influence of buffer concentration and composition, pH, running voltage and temperature on the electroosmotic mobility and electrophoretic mobilities of the model samples (rabbit liver MT and horse kidney MT) are investigated. Also the use of organic solvents as buffer modifiers and their effect on the separation of putative metallothionein isoforms is described.

Additional information

Authors: VIRTANEN V, JRC Geel (BE);BORDIN G, JRC Geel (BE);RODRIGUEZ A R, JRC Geel (BE)
Bibliographic Reference: Article: Chromatographia (1998)
Record Number: 199811320 / Last updated on: 1998-11-10
Category: PUBLICATION
Original language: en
Available languages: en