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Abstract

The results of a collaborative trial study including 29 laboratories of 13 countries applying a method based on the polymerase chain reaction (PCR) for the detection of genetically modified organisms (GMO) in food are presented. For this study various reference materials were produced that were derived from genetically modified soy beans and maize, respectively. The results of this study indicate that a correct identification of samples containing 2%GMO is achievable for both matrices, soy beans and maize,respectively. For samples containing 0.5% genetically modified soy beans the analysis of the 35S promotor resulted as well in a 100% correct classification. Due to the bigger genomic DNA of maize, the probability of encountering false negative results for samples containing 0.5% GMO is somewhat greater than for soy beans.

Additional information

Authors: LIPP M, IHCP, Food Products Unit , Ispra (IT);BRODMANN P, Kantoles Labor Basel, Basel (CH);PIETSCH K, Chemische Landesuntersuchungsanstalt Freiburg Bissierstrasse, Frieburg (DE);PAUWELS J, IRMM Ispra (IT);ANKLAM E, IHCP Food Products Unit, Ispra (IT)
Bibliographic Reference: Article : Journal of AOAC International, 1998
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