Validation of Analytical methods to Determine the Content of Aflatoxin, Ochratoxin and Patulin in Foodstuffs of Vegetable Origin
An interlaboratory comparison was conducted to evaluate the effectiveness of an immunoaffinity column clean-up HPLC method for the determination of aflatoxin M1 in milk at proposed European regulatory limits. The test portion of liquid milk was and applied to an immunoaffinity column. The column was washed with water and the aflatoxin eluted with methanol. Aflatoxin M1 was separated by reverse-phase liquid chromatography (HPLC) with fluorescence detection. Frozen liquid milk samples both naturally contaminated with aflatoxin M and blank samples for spiking, were sent to 13 collaborators in 13 different European countries. Test portions of samples were spiked at 0.05 ng/g aflatoxin M. Recoveries of aflatoxin M ranged from 42% to 94% with a mean recovery of 69%. Based on results for spiked samples (blind pairs at one level) as well as naturally contaminated samples (blind pairs at three levels) the relative standard deviation for repeatability (RSD) ranged from 7% to 18%. The relative standard deviation for reproducibility (RSD) ranged from 21% to 34%. The method showed acceptable within laboratory and between laboratory precision data for liquid mild, as evidenced by HORRAT values and met the minimum method performance criteria set by CEN.
Bibliographic Reference: EUR 18856 EN (1999) 37pp.
Availability: Available from OOPEC Sales agents
ISBN: ISBN 92-828-5926-6
Record Number: 199910598 / Last updated on: 1999-04-01
Original language: en
Available languages: en