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The granulocyte-macrophage colony forming unit assay has been widely used to investigate the pathogenic mechanism of drug induced blood disorders and for screening the potential myelotoxicity of antineoplastics. Considering the wide use of this technique, there is a need for a more standardized protocol, particularly with respect to the exposure conditions. The sensitivity of granulocyte-macrophage progenitors to three antineoplastic drugs; Etoposide, Doxorubicin and Camptothecin, measured by the GM-CFU agar assay, is described. Continuous drug treatment has been compared with short-term drug pretreatments (1, 6 and 24 hr exposure) using granulocyte-macrophage progenitors from both frsh bone marrow and from long term bone marrow culture. The continuous exposure of fresh bone marrow cells to the drugs resulted in a dose-dependant inhibition of progenitor growth.

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Authors: GRIBALDO L, ECVAM, Ispra (IT);CASATI S, ECVAM, Ispra (IT);COLLOTA A, ECVAM, Ispra (IT);PICCIRILLO M, Institute of Medical Microbiology, Milan (IT);MINEO E, Institute of Medical Microbiology, Milan (IT);PESSINA A, Institute of Medical Microbiology, Milan (IT)
Bibliographic Reference: Article: Toxicology in Vitro, 12 (1998) 39-45
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