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Abstract

The results of a collaborative study including 29 laboratories of 13 countries applying a method based on the ploymerase chain reaction (PCR) for the dtection of genetically modified organisms (GMO) in food presented. The method based on a recent publication that describes the suitability of the determination of the 35S promotor and the NOS terminator for the detection of GMO. For this study various reference materials were produced that were derived from genetically modified soy beans and maize, respectively. The results of this study indicate that a correct identification of samples containing 2% GMO is achievable for both matrices, soy beans and maize, respectively. For samples containing 0.5% genetically modified soy beans the analysis of the 35S promotor resulted as well as 100% correct classification. However, three false negative results were reported for the analysis of the NOS terminator, which due to lower sensitivity of this method. Due to the bigger genomic DNA of maize, the probability of encountering fals negative results for samples containing 0.5%GMP is somewhat greater than that of soy beans. For the blank samples (0% GMO), only two false positive results for soy beans and one for maize were reported. These results appeared as very weak signals and were most probably due to contamination of the laboratory equipment.

Additional information

Authors: LIPP M, IHCP, JRC Ispra (IT);BRODMAN P, Kantonales Labor Basel (CH);PIETSCH K, Chemische Landesuntersuchungsanstalt Freiburg (DE);PAUWELS J, IRMM, Geel (BE);ANKLAM E, IRMM, Geel (BE)
Bibliographic Reference: Article: Project 650/93/97 of the Commision on Food Chemistry, 1998
Record Number: 199910700 / Last updated on: 1999-05-08
Category: PUBLICATION
Original language: en
Available languages: en