Preparation of a certified prostate specific antigen (psa) reference material
The presented report describes the purification, characterization, ampouling, certification and stability investigation of Prostate Specific Antigen. The intended use of the PSA CRM 613 is as a calibrant within immunological test systems. The purification of prostate specific antigen was performed via anion-exchange chromatography, gel permeation chromatography and reversed phase high performance liquid chromatography. The overall yield amounts to 20-35% lyophilized salt-free protein with a purity >98% determined by the N-terminal sequencing, total hydrolysis and other methods. The purification procedure does not lead to significant degradation e.g internal peptide bond cleavage.
Bibliographic Reference: EUR 18900 EN, (1999) 56pp.
Availability: Available from OOPEC Sales agents
ISBN: ISBN 92-828-6625-4
Record Number: 199911000 / Last updated on: 1999-07-23
Original language: en
Available languages: en