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The presented report describes the purification, characterization, ampouling, certification and stability investigation of Prostate Specific Antigen. The intended use of the PSA CRM 613 is as a calibrant within immunological test systems. The purification of prostate specific antigen was performed via anion-exchange chromatography, gel permeation chromatography and reversed phase high performance liquid chromatography. The overall yield amounts to 20-35% lyophilized salt-free protein with a purity >98% determined by the N-terminal sequencing, total hydrolysis and other methods. The purification procedure does not lead to significant degradation e.g internal peptide bond cleavage.

Additional information

Authors: PARFAIT R, Bureau Raymond G.Parfait sarl, Dammartine en Goele (FR);MAST W P, SERTAC GmbH Goettingen (DE);VOLK M, SERTAC GmbH Goettingen (DE);LYNCH S, SEAMUS LYNCH, Warwickshire (DE);VANDEKERCHOVE J, University of Gent, Gent (BE);CARAYON P, Universite d'Aix, Marseille (FR);CHRISTOFIDIS I, Immunoassay Quality Control Lab, Athens (GR)
Bibliographic Reference: EUR 18900 EN, (1999) 56pp.
Availability: Available from OOPEC Sales agents
ISBN: ISBN 92-828-6625-4
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