Community Research and Development Information Service - CORDIS

Abstract

This article shows the effect of 44 different metal ions on the activity of the reverse transcripts of the human immunodeficiency virus was investigated in vitro. For this study, the RT activity assay was carried out by means of an enzyme-linked immunosorbent assay kit, using the template/primer hybrid poly (A) oligo(dT)15, which required some modifications. The results are indicative of enzyme thermolability, which is higher in the absence of substrates. The effect of metal ions on RT activity was tested using two different metal salt concentrations.

Additional information

Authors: SABBIONI E, EI, JRC-Ispra (IT);BLANCH N, EI, JRC-Ispra (IT);BARICEVIC K, EI, JRC-Ispra (IT);SERRA M A, EI, JRC-Ispra (IT)
Bibliographic Reference: Article: Biological Trace Element Research, 68 (1999), 107-119
Record Number: 199911223 / Last updated on: 1999-08-27
Category: PUBLICATION
Original language: en
Available languages: en