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Abstract

This report describes the results of a method development/optimization study for fumonisin B1 (FB1) and fumonisin B2 (FB2) in five maize based matrices Two critical steps have been identified in the method for analysis of fumonisin in maize flour, cornflakes, extruded product, muffins and infant formula. The extraction step of fumonisins from the materials and the clean-up step of the crude extracts were demonstrated to be critical to the performance of the methods. Test portion size, extraction solvent volume and extraction mode were demonstrated to have minor or no effects on the performance of the methods. The use of immunoaffinity columns was found to be essential for the purification of cornflakes, muffins and infant formula extracts. The use of ACN+water, 1+1,v+v compared with MeOH+water, 3+1, v+v gave higher fumonisins recoveries for all but infant formula matrices. However, phase separation occurred during the extraction step, making this solvent mixture inappropriate. This report describes the results of a method development/optimization study for fumonisin B1 (FB1) and fumonisin B2 (FB2) in five maize based matrices Two critical steps have been identified in the method for analysis of fumonisin in maize flour, cornflakes, extruded product, muffins and infant formula. The extraction step of fumonisins from the materials and the clean-up step of the crude extracts were demonstrated to be critical to the performance of the methods. Test portion size, extraction solvent volume and extraction mode were demonstrated to have minor or no effects on the performance of the methods. The use of immunoaffinity columns was found to be essential for the purification of cornflakes, muffins and infant formula extracts. The use of ACN+water, 1+1,v+v compared with MeOH+water, 3+1, v+v gave higher fumonisins recoveries for all but infant formula matrices. However, phase separation occurred during the extraction step, making this solvent mixture inappropriate. This report des

Additional information

Authors: VISCONTI A, Institute for Toxins Mycotoxins from Plant parasites, Bari (IT);SOLFRIZZO M, Institute for Toxins Mycotoxins from Plant parasites, Bari (IT);DE GIROLAMO A, Institute for Toxins Mycotoxins from Plant parasites, Bari (IT);BOENKE A, Standard, Measurements and Testing Programme, Brussels (BE)
Bibliographic Reference: EUR 19096 EN (1999), 163pp.
Availability: Available from OOPEC Sales agents
ISBN: ISBN 92-828-8018-4
Record Number: 199911653 / Last updated on: 1999-11-29
Category: PUBLICATION
Original language: en
Available languages: en
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