Community Research and Development Information Service - CORDIS

Abstract

The Institute for Reference Materials and Measurements (IRMM) and the International Federation for Clinical Chemistry and Laboratory Medicine (IFCC) have, according to a co-operation agreement, certified a reference material (CRM) under the name and number IRMM/IFCC-451. This report describes the quantification of cortisol in a panel of 34 native serum samples obtained from 18 female and 16 male blood donors. The concentrations of cortisol in the serum samples range from 82.7 to 764.2 nmol/L. The certification of the sera is based on the results obtained in two laboratories using two reference measurement procedures based on the primary ratio method: isotope dilution gas chromatography/mass spectrometry. It is the intention that the reference serum panel should be used to standardize cortisol test systems. It will mainly be distributed to manufacturers and is the key factor in a pilot study for validation/verification of routine procedures for measuring cortisol through comparison with the reference methods. The different routine methods can be tested in terms of sensitivity, specificity and trueness.

Additional information

Authors: (BE), ;IRMM-GEEL, ;REFERENCE INSTITUTE OF BIOANALYSIS BONN (DE), ;UNIVERSITY OF GENT (BE), ;FACULTY OF PHARMACEUTICAL SCIENCES,
Bibliographic Reference: EUR 19007 EN (1999), 13pp.
Availability: Available free of charge from by Documentation Unit JRC Ispra (IT)
Record Number: 199911730 / Last updated on: 2001-06-12
Category: PUBLICATION
Original language: en
Available languages: en