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  • Standard Operation Procedure for the Determination of Aflatoxins in Various Food Matrices by Immunoaffinity Clean-up and Thin Layer Chromatography


This method can be applied to the determination of aflatoxins in fig paste, pistachios, peanut butter, paprika powder and corn. The limit of detection for visual quantification of the method has been demonstrated to be better than 1 ug/kg for each aflatoxin, while the limit of quantificationby a TLC-scanner has been demonstrated to be better than 0.5ug/kg for each aflatoxin. The method involves extraction of a test portion of fig paste, corn or paprika powder with a solvent solution while for peanuts, almonds and pistachios an additional non-polar solvent is used to break the fat phase of the matrx. The sample extract is filtered, diluted with water to a specified solvent concentration, and applied to an immunoaffinity column containing antibodies specific to aflatoxins B1, B2, G1 and G2. The aflatoxins are removed from the immunoaffinity columns with methanol and the eluate is then evaporated. The dried aflatoxins are re-dissolved in a defined volume of acetone/t-butylmethylether, separated by a thin layer chromatography and then quantified either visually under UV-light or with TLC-plate scanner.

Additional information

Authors: STROKA J, IHCP, JRC-Ispra (IT);ANKLAM E, IHCP, JRC-Ispra (IT);OTTERDIJK R, Cheetah Zambia Ltd, Lusaka (ZA)
Bibliographic Reference: EUR 19027 EN (1999), 16pp.
Availability: Available free of charge by Documentation Unit JRC Ispra (IT)
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