Validation of an Analytical Method to Determine the Content of Aflatoxin in Animal Feeding Stuff
An interlaboratory comparison was carried out to evaluate the effectiveness of an immunoaffinity column clean-up HPLC method for determination of aflatoxin B1 in animal feed at concentrations of 1 and 5 ng/g. The test portion of the sample was extracted with aceton-water. The sample extract was filtered, diluted with water and applied on an immunoaffinity column. The column was washed with water to remove any interfering matrix components while the purified aflatoxin B1 was subsequently eluted with methanol. The following separation and determination of the aflatoxin B1 was performed by reverse-phase high performance liquid chromatography and detected by fluorescence after post column derivatisation involving bromination. PCD was achieved with either an electrochemical cell and addition of bromide to the mobile phase or with pyridinum hydrobromide perbromide. The feeding stuff samples, both spiked and naturally contaminated with aflatoxins, were sent to 23 laboratories in 12 different European and 2 non-European countries.
Bibliographic Reference: EUR 19058 EN (2000), 25pp.
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Record Number: 200011891 / Last updated on: 2000-03-31
Original language: en
Available languages: en