Community Research and Development Information Service - CORDIS


Determination of trace amounts of fatty acids in edible oils by capillary gas-liquid chromatography

Funded under: FP5


The effect of using different GLC hardware on the quantification of low concentrations of fatty acids was studied. A fused silica capillary column was operated in two different chromatographs (A and B) which were interfaced to three different chromatographic data systems to process the FID signals (systems A, B1 and B2). A test routine was developed that allowed the proper selection of peak processing parameters for the automatic recognition and integration of fatty acids occurring at trace levels. However, agreement of analytical results between the three analytical systems was not satisfactory, components at concentrations < 0.10 g / 100 g could not be quantified with high reliability, although the same capillary column and identical sample solutions were used (quasi-repeatability conditions). Even for major fatty acids deviations up to 1.0 g / 100 g of total fatty acids were noted, which could only be attributed to the use of different chromatographic hardware. Attention should be paid to these technical restrictions when formulating product specifications based on fatty acid profile related parameters.

Additional information

Authors: BUCHGRABER M, IHCP Institute, Unit J02, Ispra (IT);ULBERTH F, IHCP Institute, Unit J02, Ispra (IT);ANKLAM E, IHCP Institute, Unit J02, Ispra (IT)
Bibliographic Reference: Article: Journal of the American Oil Chemists' Society - AOCS Press
Availability: Journal of the American Oil Chemists' Society - AOCS Press
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