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Abstract

The aim of the study was twofold. Firstly, to select, stabilize and bottle a suitable cocoa butter (CB) sample and to demonstrate that packaging and storage does not affect CB composition, and secondly, to verify that methods used by the participants are fit-for-purpose and sufficient agreement of results between participants can be achieved.

Stabilizing the CB sample with tert. Butylhydroquinone (250 mg/kg) and packaging in inert atmosphere in amber glass ampoules resulted in homogeneous and stable lots of the preliminary test material.

Analytical precision of chromatographic methods to determine individual triglycerides (TGs) was acceptable (RSD(R) < 5%) for the main components POP, POS and SOS with analyte levels > 15 g/100 g, for analyte levels between 1 to 5 g/100 g the RSD(R) was < 30% and for TGs at levels < 1 g/100 g precision was beyond the acceptance criterion (RSD(R) > 30%). Outlier removal improved precision of components occurring at levels between 1 to 5 g/100 g to RSD(R) < 20% (with one exception), but no further improvement was achieved for analytes < 1 g/100 g.

The results indicate that a cocoa butter based reference material, which is sufficiently homogeneous and stable, can be produced. Furthermore, the precision obtained by different analytical methods would justify to assign certified values for the major triglyceride fractions of cocoa butter. tter.

Additional information

Authors: BUCHGRABER M, Department of Dairy Research and Bacteriology, University of Agricultural Sciences, Vienna (AT);ULBERTH F, Department of Dairy Research and Bacteriology, University of Agricultural Sciences, Vienna (AT);LIPP M, European Commission, DG JRC, Institute for Health and Consumer Protection, Ispra (IT);ANKLAM E, European Commission, DG JRC, Institute for Health and Consumer Protection, Ispra (IT)
Bibliographic Reference: EUR 19565 EN (2000) 51 pp.
Availability: Available free of charge from Documentation Service, DG Research
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