Community Research and Development Information Service - CORDIS

Abstract

This report describes the preparation and certification of a new set of six soya bean powder CRM's (IRMM-410R) with different mass fractions of genetically modified Roundup Ready (TM) dry powder (Certified reference materials SB-0, SB-0.1, SB-0.5, SB-1, SB-2 and SB-5). The CRM's were produced by IRMM on behalf of Fluka Chemie AG in replacement of IRMM-410, produced in 1999 in the frame of a project of the Environment Institute (EI) of the Joint Research Centre of the European Commission (Ispra, Italy) aiming at the validation of the polymerase chain reaction (PCR) screening method for the detection of genetically modified plants. The Roundup Ready (TM) concentration of IRMM-410R was verified at Kantonales Laboratorium Basel-Stadt in Switzerland. The CRM's are available in the form of glass bottles containing 1 g of soya bean powder packed under argon atmosphere. Seeds of biologically grown non-modified soya bean and Roundup Ready (TM) GMO soya bean were heated at 90 degrees Celsius for 17 to 35 hours in order to reduce enzymatic activities and ground and dried afterwards. The materials were prepared by quantitative mixing in aqueous suspension of non-modified soya bean powder and 100% Roundup Ready (TM) GMO soya bean powder, and subsequent freeze-drying, grinding, homogenization and bottling. The pre-filled bottles were submitted to a supplemental primary vacuum drying for around two hours and closed after filling with pure argon. It is recommended to use samplings not smaller than 100 mg. Unopened bottles can be stored at room temperature, preferably in the dark. Once opened, bottles should be stored dry and cool.

Additional information

Authors: BRODMANN P, Kantonales Laboratorium Basel-Stadt, Basel (CH);VAN DEN EEDE G, EC-JRC-Environmental Institute, Ispra (IT);ANKLAM E, EC-JRC-Environmental Institute, Ispra (IT);PAUWELS J, EC-JRC-Institute of Reference Materials and Measurements, Geel (BE);SCHIMMEL H, EC-JRC-Institute of Reference Materials and Measurements, Geel (BE);KRAMER G N, EC-JRC-Institute of Reference Materials and Measurements, Geel (BE);LE GUERN L, EC-JRC-Institute of Reference Materials and Measurements, Geel (BE);TRAPMANN S, EC-JRC-Institute of Reference Materials and Measurements, Geel (BE)
Bibliographic Reference: EUR 19573 EN (2000) 6 pp.
Availability: Available free of charge from JRC Ispra
ISBN: ISBN 92-828-9639-0
Record Number: 200012172 / Last updated on: 2001-06-12
Category: PUBLICATION
Original language: en
Available languages: en