Community Research and Development Information Service - CORDIS

Abstract

Although the revised protocol attached to this report might require further refining, it could serve as a basis for further evaluation. It is designed to obtain data that are more consistent and for which the number of false positive results should be drastically reduced. Refinements might be introduced, for instance, to chose the optimum range of GMO (Genetically Modified Organisms) concentrations for which the test is applicable. This is based on the observation that apparently no single method can be applied for a large range (>5%) of GMO concentrations.

From the second part of the project, we thus conclude that in contrast to the qualitative method described above, the quantitative method did not produce consistent results. This could be due to the following:

-Participants disliked the complex protocol.
-Participants were keener to use sophisticated equipment.
-Participants lacked hands-on experience.
-The GM-materials produced in two different series using the same protocols have been found to be inconsistent, i.e. a 1% GMO preparation from the second series showed the same results as a 2% GMO preparation from the first series. An explanation is provided in this report.
-Based on these findings new and consistent materials have been prepared by the Institute for Reference Materials and Measurements of the JRC. They are currently used in other validation studies.

This study has also shown that the choice of the DNA molecules and primer sequences used for this type of quantification are crucial and that their performance should be optimised for each matrix considered.

Based on the above, it can be concluded that more research is needed and that more data are required to develop and validate quantitative methods that have a high level of accuracy and precision around the 1% threshold level.

Additional information

Authors: ANKLAM E, DG-JRC Institute for Health and Consumer Protection, Ispra (IT);EYQUEM F, DG-JRC Institute for Health and Consumer Protection, Ispra (IT);LIPP M, DG-JRC Institute for Health and Consumer Protection, Ispra (IT);VAN DEN EEDEN G, DG-JRC Institute for Health and Consumer Protection, Ispra (IT)
Bibliographic Reference: EUR: 19676 EN (2000), pp40pp, Euro: free of charge
Availability: Available from Documentation Service JRC Ispra (IT) Fax: +39-0332-7858182
ISBN: ISBN: N/A
Record Number: 200013082 / Last updated on: 2001-01-31
Category: PUBLICATION
Original language: en
Available languages: en