Control of appropriate heat treatment of animal waste using the ELISA technique: Validation and calibration of an analytical method
The applicability of an ELISA based method (pork assay) for the determination of appropriate heat treatment of animal meal has been demonstrated by performing a validation study of the method and by calibrating the test kit using samples from a commercial rendering plant. The evaluation of the results of the validation study encompassing 21 laboratories from 12 European countries revealed the robustness of the method, a sufficient sensitivity to detect a deviation of the processing conditions from the regulation and a low standard deviation of the data. Additionally, the test kit has been validated by performing trials according to the factorial design approach in laboratory autoclave and by experiments in a commercial rendering plant. We conclude from these trials, that a R-value of 2 indicates proper heat treatment of the animal meal.
Bibliographic Reference: Oral delivery given at: Feed Production Conference. Hosted by: Professor Piva, Universita die Piacenza. Held at: Picenza (IT) 27-28 November, 2000
Record Number: 200013100 / Last updated on: 2001-02-23
Original language: en
Available languages: en