ECVAM/2000/22 Inhibition of CFU-E/BFU-E by 3' -azido-3'-deoxythymidine, chlorpropamide and protoporphirin IX zinc (II): a comparison between direct exposure of progenitor cells and long-term exposure of bone marrow cultures
Erithropoiesis occurs in two stages: proliferation amplifies cell number, and differentiation stimulates the acquisition of the functional properties of red blood cells. The erythroid colony- forming unit (CFU-E) amplifies the differentiation process in response to erythropoietic stress in vitro, whereas the burst forming unit (BFU-E), which is not particularly sensitive to erythropoietin stimulation, gives rise to the CFU-E and, when stimulated, produces morphologically- identifiable erythroid colonies. The aim of this work was to evaluate the toxic effects of the antiviral agent, 3'-azido-3'-deoxythymidine (AZT), the antidiabetic drug, chlorpropamide (CLP), and the heme-analogous compound, protophorphirin IX zinc (II) (ZnPP), on the proliferation of erythroblastic progenitors by using human cord blood cells and murine progenitors from long-term bone marrow cultures.
Bibliographic Reference: Article published in:Toxicological Sciences, 58 pp96-101
Record Number: 200013105 / Last updated on: 2001-02-23
Original language: en
Available languages: en