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Protein and RNA synthesis in the fish cell lines BF-2, RTP and CHSE 214, were determined from 3H- phenylalanine and -uridine incorporation, respectively. Differences in protein synthesis rates, BF-2 > RTP > CHSE 214, were attributable to RNA translation efficiency (mg. protein synthesised ìg-1 RNA day-1). The most translationally efficient RNA (BF-2 cells) possessed the fastest turnover. BF-2 cells also utilised lower cost exogenous nucleotide salvage more than the least translationally efficient CHSE 214 cells. Nucleotide supply, between intracellular synthesis and exogenous salvage, may be exploited to maintain the fixed energetic cost of RNA synthesis.

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Authors: HOULIHAN D.F, Rowett Research Institute, Aberdeen (UK);PALMER R.m, University of Aberdeen, Department of Zoology (UK);SMITH R.W, JRC-Environment Institute, Ispra (IT)
Bibliographic Reference: An article published in: Journal of Compehensive Physiology B (2000) 170 pp.135-144
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