Community Research and Development Information Service - CORDIS

Abstract

In this paper we describe the validation and utilization of an DELFIA (Reg.) immunoassay for detecting polychlorinated biphenyls (PCBs) in animal fat. The immunoassay incorporates a rapid sample processing protocol that has been optimised to detect concentrations of PCBs in animal fat at or above 200 ng/g. The limit of detection of the assay was less than 50 ng/g. When the action level for the DELFIA assay was set at 100 ng/g to detect samples having 200 ng/g or more PCBs, the false negative rate was estimated at 0.2 %. By utilizing the DELFlA assay system to select for positive samples and GC/MS analysis for conformation aI1alysis, an accurate and cost-effective program has been established for the high throughput detection of PCBs in animal products.

Additional information

Authors: JABOREK-HUGO S, JRC, Institute for Health and Consumer Protection, Food Products and Consumer Goods Units, Ispra (IT);VON HOLST C, JRC, Institute for Health and Consumer Protection, Food Products and Consumer Goods Units, Ispra (IT);ANKLAM E, JRC, Institute for Health and Consumer Protection, Food Products and Consumer Goods Units, Ispra (IT);ALLEN R, Hybrizyme, Raleigh, North Carolina (US);STEWART T, Hybrizyme, Raleigh, North Carolina (US);WILLEY J, Hybrizyme, Raleigh, North Carolina (US)
Bibliographic Reference: An article published in: Food Additives and Contaminants (2001), Vol.18. No.2 pp.121-127
Availability: An article published in: Food Additives and Contaminants (2001), Vol.18. No.2 pp.121-127
Record Number: 200013367 / Last updated on: 2001-06-27
Category: PUBLICATION
Original language: en
Available languages: en