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Cytosolic proteins were studied by isoelectric focusing (IEF) and two-dimensional electrophoresis (2-DE) of samples from HaCaT keratinocytes challenged with Ni(II) . Proteins with isoelectric points of 6.9, 7.7 and 8.5 were visualized in blots using three different staining procedures only in Ni-treated cells. Two of them were unequivocally identified after 2-DE, N-terminal sequencing and database search as phosphoglycerate kinase and annexin II. The involvement of these proteins in cellular functions and possible implications in the irritative and allergic phenomena caused by Ni in the skin( i.e. Ni-induced allergic contact dermatitis), as well as their possible use as biomarkers are discussed.

Additional information

Authors: ACEVEDO F, National Institute for Working Life, Solna (SE);VESTERBERG O, National Institute for Working Life, Solna (SE);MIGUEL-NGEL S, Environment Institute, JRC, Ispra (IT);ERMOLLI M, Environment Institute, JRC, Ispra (IT);CLERICI L, Environment Institute, JRC, Ispra (IT)
Bibliographic Reference: An article published in: Elsevier: TOXICOLOGY, 159 (2001) pp.33-41
Record Number: 200013383 / Last updated on: 2001-06-27
Original language: en
Available languages: en