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Abstract

Cytotoxicity (MTT test) and cell viability (CFE) along with metal intracellular accumulation were studied in human HaCaT keratinocytes challenged with nickel, cobalt or chromium within a SCA project entitled "Indicators of metal sensitisation". ED50 values were calculated, being Cr(VI)<Co(II)<Ni(II). Metals accumulated mainly in the cytosol, the percentage being Ni(II)>Co(II)>Cr(VI), and were concentrated with respect to the external dose 30, 9,5 and 400 times, respectively. These findings are discussed in relation to the cytotoxic mechanisms underlying metal-induced allergic contact dermatitis.

Additional information

Authors: ERMOLLI M, JRC-Environment Institute, Environmental Impact Unit, Ispra (IT);MENN C, JRC-Environment Institute, Environmental Impact Unit, Ispra (IT);POZZI G, JRC-Environment Institute, Environmental Impact Unit, Ispra (IT);MIGUEL-NGEL S, JRC-Environment Institute, Environmental Impact Unit, Ispra (IT);CLERICI L.A, JRC-Environment Institute, Environmental Impact Unit, Ispra (IT)
Bibliographic Reference: An article published in: Elsevier: TOXICOLOGY, 159 (2001) pp.23-31
Record Number: 200013384 / Last updated on: 2001-06-27
Category: PUBLICATION
Original language: en
Available languages: en
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