Nickel, Cobalt and Chromium-induced Cytotoxicity and Intracellular Accumulation in Human HaCaT Keratinocytes
Cytotoxicity (MTT test) and cell viability (CFE) along with metal intracellular accumulation were studied in human HaCaT keratinocytes challenged with nickel, cobalt or chromium within a SCA project entitled "Indicators of metal sensitisation". ED50 values were calculated, being Cr(VI)<Co(II)<Ni(II). Metals accumulated mainly in the cytosol, the percentage being Ni(II)>Co(II)>Cr(VI), and were concentrated with respect to the external dose 30, 9,5 and 400 times, respectively. These findings are discussed in relation to the cytotoxic mechanisms underlying metal-induced allergic contact dermatitis.
Bibliographic Reference: An article published in: Elsevier: TOXICOLOGY, 159 (2001) pp.23-31
Record Number: 200013384 / Last updated on: 2001-06-27
Original language: en
Available languages: en