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Abstract

An experimental design procedure was used to investigate the effects of some operating parameters on the supercritical fluid extraction of carotenoids Î-carotene, Î-cryptoxanthin and zeaxanthin from Spirulina Pacifica algae. a carotenoid-rich dietary product. Variables tested were temperature and pressure of the supercritical fluid, dynamic extraction time and percentage of ethanol added as the modifier. Each variable was tested at three levels; 31 experiments were performed in random order. Analyses of the extracts were performed by high-performance liquid chromatography with UV-Vis photodiode array detection. Analytical responses (chromatographic peak areas) were processed by using a stepwise multiple regression analysis, in order to find polynomial functions describing the relationships between variables and responses. For all the analytes the experimental conditions providing the highest extraction yield inside the experimental domain considered were found. Supercritical fluid extraction results obtained in these conditions were compared with those obtained by performing solvent extraction in order to evaluate the effectiveness of the supercritical fluid extraction procedure.

Additional information

Authors: CARERI M, Dipartimento di Chimica Generale ed Inorganica, Chimica Analitica, Chimica Fisica, Universita degli Studi di Parma (IT);FURLATTINI L, Dipartimento di Chimica Generale ed Inorganica, Chimica Analitica, Chimica Fisica, Universita degli Studi di Parma (IT);MANGIA A, Dipartimento di Chimica Generale ed Inorganica, Chimica Analitica, Chimica Fisica, Universita degli Studi di Parma (IT);ANKLAM E, EC, DG-JRC, Institute for Health and Consumer Protection, Ispra (IT);THEOBALD A, EC, DG-JRC, Institute for Health and Consumer Protection, Ispra (IT);VON HOLST C, EC, DG-JRC, Institute for Health and Consumer Protection, Ispra (IT)
Bibliographic Reference: An article published in: Elsevier Science, Journal of Chromatography A, 912 (2001) pp.61-71
Record Number: 200013503 / Last updated on: 2001-07-24
Category: PUBLICATION
Original language: en
Available languages: en