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The preliminary results on an inter-laboratory trial on the detection of genetically modified organisms (GMO) are presented. The method applied is based on the detection of modified DNA using the polymerase chain reaction (PCR) for amplification. The amplified fragments analysed are derived from the 35S promotor and the NOS terminator used for modification and are present in 26 from the 28 GMOs currently already approved or under approval by the competent authorities. This method fits as a screening method indicating the presence of GMO in food. However, it does not allow an identification of the kind of GMO present in the samples. Samples of soybean and maize flour containing 0%, 0.1 %, 0.5% and 2% GMO had been prepared for this study and are also already commercially available. In this paper the combined results from 27 laboratories are presented, indicating that on average the probability of false positive or false negative results is only about 1% for soybeans and below 5% for maize.

Additional information

Authors: LIPP M, EC-JRC, Institute for Health and Consumer Protection, Food Product Unit, Ispra (IT);ANKLAM E, EC-JRC, Institute for Health and Consumer Protection, Food Product Unit, Ispra (IT);BROADMANN P, Kantonales Labor, Freiburg (DE);PIETSCH K, Chemische Landesuntersuchungsanstalt , Freiburg (DE);PAUWELS J, EC-JRC, Institute of Reference Materials and Measurements, Geel (BE)
Bibliographic Reference: An article published in: Elsevier Science Ltd, Food Control 10 (1999), pp.379-383
Record Number: 200013510 / Last updated on: 2001-07-24
Original language: en
Available languages: en