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Abstract

CdCI2 is a well-known toxic compound for the kidney in vivo and in vitro. We report here part of the results of an ECV AM (the European Centre for the Validation of Alternative Methods) contract study, aimed to establish and assess several flow cytometric and confocal microscopic endpoints for use in an in vitro nephrotoxicity model. Three renal tubule cell lines, OK, LLC-PK I, and MDCK were exposed for I, 5 and 24 hours to 25 microM and 100 microM CdCI2. The results obtained for mitochondrial membrane potential showed a decrease in all the cell lines after 5 hours of treatment with both CdCI2 concentrations. In some cases, this decrease was detected by flow cytometry after a I-hour exposure. On the contrary, intracellular Ca2+ increased in a time-dependent and concentration-dependent fashion. This increase was especially high in the MOCK cell line after a 24-hour exposure to 100 microM CdCI2. However, cell viability was not affected by 25 microM CdCI2. Our results demonstrate early changes in mitochondrial membrane potential and cytoplasmic Ca2+ levels in renal tubular epithelial cell lines treated with CdCI2.

Additional information

Authors: ALVAREZ-BARRIENTOS A, Flow Cytometry and Confocal Microscopy Unit, Complutense University, Madrid (ES);NIETO CASTILLO R, Flow Cytometry and Confocal Microscopy Unit, Complutense University, Madrid (ES);MORENO MORENO A.B, Flow Cytometry and Confocal Microscopy Unit, Complutense University, Madrid (ES);O'CONNOR J.E, Department of Biochemistry and Molecular Biology, University of Valencia (ES);PRIETO P, ECVAM, Institute for Health & Consumer Protection, EC-JRC, Ispra(IT)
Bibliographic Reference: An oral report given at: INVITOX 2000. Organised by: European Society of Toxicology In-Vitro (ESTIV). Held in: El Campello, Alicante (ES), 24-28 October 2000
Record Number: 200013522 / Last updated on: 2001-07-25
Category: PUBLICATION
Original language: en
Available languages: en