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Abstract

An electrochemical study of the complex properties of the C-terminal peptidic fragment Lys-Cys-Thr-Cys-Cys-Ala {56-61} of mouse liver MT 1 in the simultaneous presence of cadmium and zinc has been performed at pH 8.0. Even using a high resolution technique as square wave voltammetry, rather involved voltammetric signals with highly overlapping peaks were obtained.

In order to reach a proper interpretation of the voltametric data, a multivariate curve resolution with alternating least squares optimisation (MCR-ALS) method has been applied. MCR-ALS allows one to obtain both the unitary voltamogram for each electrochemical process and the concentration profiles along the experiment. The formation of ternary mixed-metal complexes, with higher stoichiometries than those of the binary Cd-peptide and Zn-peptide is reported. Results are compared with those obtained from previous studies but without multivariate data analysis, and with some results concerning metallothioneins and their alpha and beta-domains. .

Additional information

Authors: DIAZ-CRUZ M.S, EC-JRC, Institute for Reference Materials and Measurements, Geel (BE);ESTEBAN M, EC-JRC, Institute for Reference Materials and Measurements, Geel (BE);RODRGUEZ A.R, EC-JRC, Institute for Reference Materials and Measurements, Geel (BE)
Bibliographic Reference: An article published in: Elsevier, Analytica Chimica Acta, 428 (2001), pp.285-299
Record Number: 200013595 / Last updated on: 2001-07-30
Category: PUBLICATION
Original language: en
Available languages: en