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  • Standard Operation Procedures for the Determination of Release of Di-Isononylphthalate (DINP) in Saliva Simulant from Toys and Childcare Articles using a Head Over Heels Dynamic Agitation Device


Phthalates are commonly used as plasticisers in soft PVC articles such as toys and childcare articles, which may contain up to 50% of them. During use of the articles by babies, these plasticisers may leach into the saliva and consequently expose them to phthalates. Considering the low body weight and the age of the babies, the exposure to phthalates should not exceed a tolerable daily intake. Previous in-vivo studies on human volunteers (Dutch Consensus Group) using standard PVC disks containing di-isononylphthalate (DINP) as plasticiser have indicated the range of release that could be expected in-vivo for DINP on standard materials. Thus there is now a need to develop in-vitro mechanically based methodologies to test toys and childcare articles which will be able to simulate the range of releases observed in vivo.

The methods allow the identification and quantification of DINP in artificial saliva after mechanical agitation with plasticised PVC. A "head over heels" device was developed based on a rotational agitation principle originating in the Netherlands (Nutrition and Food Research Institute, TNO). The method was optimised by the European Commission Joint Research Centre (JRC) and validated with Member States laboratories. DINP was taken as model since it is currently the most commonly used phthalate in PVC toys and childcare articles.

Additional information

Authors: SIMONEAU C, EC-JRC, Institute for Health and Consumer Protection Food Products Unit, Ispra (IT);RIJK R, Packaging Research Department TNO Nutrition and Food Research Institute, Zeist (NL)
Bibliographic Reference: EUR 19899 EN (2001), pp.20. Euro: Free of charge
Availability: Available free of charge from JRC Public Relations Unit. Ispra (IT) Fax: +39-0332-785409
Record Number: 200013647 / Last updated on: 2001-08-24
Original language: en
Available languages: en