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Abstract

From 1996-2000 Zebet has coordinated the ECVAM (European Centre for the Validation of Alternative Methods) pre-validation and validation study of three embryotoxicity tests (embryonic stem cell test = EST, whole embryo culture = WEC, and the micromass assay = MM). The three in vitro embryotoxicity tests proved applicable to testing a diverse group of chemicals compromising different embryotoxic potential (none, weak/moderate, strong). The concordance between the embryotoxic potential derived from the in vivo data and from the in vitro data was very good. Applying the PM of the EST to in vivo data, in 78% of the experiments chemicals were classified correctly. For the MM and the WEC test the PMs provided 71% and 80% correct classification respectively and more importantly a predictivity of 100% was obtained with strong embryotoxic chemicals in each of the three assays.

Additional information

Authors: SPIELMANN H, BgVV, Berlin (DE);GENSCHOW E, BgVV, Berlin (DE);SCHOLZ G, BgVV, Berlin (DE);BROWN N A, St George�s Medical School, Department of Anatomy, London (GB);PIERSMA A H, RIVM, Laboratory of Health Effects, Bilthoven (NL);BRADY M, AstraZeneca, Central Toxicology Laboratory, Macclesfield (GB);CLEMANN N, Novartis Pharma, Basel (CH);HUUSKONEN H, KTL, National Public Health Institute, Kuopio (FI);PAILLARD F, Sanofi-Synthelabo Recherche, Gargenville (FR);BREMER S, JRC, Institute for Health and Consumer Protection, Ispra (IT);BECKER K, Schering, Biological Development and Experimental Toxicology, Berlin (DE)
Bibliographic Reference: An article published in: ALTA, Vol.29 (2001), pp.301-303
Record Number: 200114034 / Last updated on: 2001-11-14
Category: PUBLICATION
Original language: en
Available languages: en