Community Research and Development Information Service - CORDIS


A simple and rapid gas chromatographic (GC) method was developed for the detection of cocoa butter equivalents (CBEs) in cocoa butter (CB). It is based on the use of a 5 m nonpolar capillary column for the separation of the main triglycerides of CB according to their acyl/carbon numbers. The GC procedure was optimised to avoid thermal degradation of the triglycerides. By computing the ratio C54/C50 and (C54/C50) x C52 and by 2-dimentional plotting of these values, authentic CB samples were clearly distinguished from samples containing various CBEs. The detection of little as 1% CBE in CB (corresponding to about 0.3% CBE in chocolate) in a model system was shown to be possible. Under real conditions, for a wide range of CBs, about 2.5% CBEs in CB mixtures, which corresponds to around 1% in chocolate; this value is far below the maximum level of 5% CBEs allowed to be added to chocolate.

Additional information

Authors: BARCAROLO R, JRC, Institute for Health and Consumer Protection, Ispra (IT);ANKLAM E, JRC, Institute for Health and Consumer Protection, Ispra (IT)
Bibliographic Reference: An article published in: Journal of AOAC International, Vol.84, No.5 (2001), pp.1485-1489
Record Number: 200114202 / Last updated on: 2002-01-02
Original language: en
Available languages: en