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Abstract

Experiments were conducted in a laboratory autoclave and in a commercial rendering plant to set a limit for the R-value of an enzyme-linked immunosorbent assay (ELISA) to prove that animal meal had been sufficiently heat-treated. The immunoassay method is currently the only technique used to check for appropriate sterilisation of animal meal. The results of these experiments were statistically assessed using operation characteristic curves and confirmed that insufficiently heat-treated animal meal has an R-value greater than 2. Results of the trials also demonstrated that the main parameters of sterilisation, temperature and duration strongly influence the R-value, thus indicating that deviation from the target sterilisation conditions is verifiable by the ELISA method.

Additional information

Authors: VON HOLST C, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT);ANKLAM E, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT);UNGLAUB W, Staatliches Tieraertzliches Untersuchungsamt Aulendorf-Centre for Diagnostic, Aulendorf (DE)
Bibliographic Reference: An article published in: Journal of AOAC International, Vol 84, No 6, (2001), pp. 1793-1798
Record Number: 200214487 / Last updated on: 2002-03-28
Category: PUBLICATION
Original language: en
Available languages: en