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The aim of this study was to explore the possibility of distinguishing between eye irritants (I; EU risk phrases R36 and R41) and non-irritants (NI) by using in vitro endpoints of the hen's egg test on the chorioallantoic membrane (the HETCAM test) and the neutral red uptake (NRU) test. Prediction models were derived by applying binary logistic regression to the in vitro data for these endpoints, which were taken from the report of a German validation study on the use of the HETCAM and 3T3 NRU tests as alternatives to the Draize eye irritation test. Whereas the validation study led to the conclusion that the combined use of the two tests enables a satisfactory discrimination between severe (R41) and non-severe (NI, R36) eye irritants, the results of the present study indicate that the two in vitro tests can also be used to discriminate between non-irritants (NI) and irritants (R36 and R41).

Additional information

Authors: WORTH A P, European Commission, Joint Research Centre, Institute for Health & Consumer Protection, Ispra (IT);CRONIN M T D, School of Pharmacy and Chemistry, Liverpool John Moores University, Liverpool (GB)
Bibliographic Reference: An article published in: In Vitro & Molecular Toxicology, Volume 14 Number 3, (2001), pp. 143-156
Record Number: 200214491 / Last updated on: 2002-03-28
Original language: en
Available languages: en
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