Development of new analytical procedures for selenium speciation in selenium-enriched yeast material
Selenium (Se) is a necessary component in the human diet, but is both essential and toxic depending on its chemical form and composition, and has the narrowest tolerance band of any element. Therefore the demand for accurate and sensitive methods for Se speciation in nutritional supplements has increased. This short paper describes work done to develop a sequential extraction procedure for the complete solubility and the recovery of Se present in the material. The fractionation of Se in yeast extracts is achieved by preparative size-exclusion chromatography. Anion-exchange chromatography with ICP-MS detection is developed to monitor the speciation of Se in low-molecular weight Se fractions. A method based on SDS-PAGE and ETV-ICP-MS for the separation and determination of Se-containing proteins has been newly applied.
Bibliographic Reference: An oral report given at: Seventh International Symposium on Hyphenated Techniques in Chromatography (HTC-7) Organised by: Royal Flemish Chemical Society (KCCV) Held at: Brugge (BE), 6-8 February 2002
Record Number: 200214532 / Last updated on: 2002-04-03
Original language: en
Available languages: en